TB-500 dosing is bigger doses, fewer pins than BPC-157, 2 mg twice a week, not 250 mcg daily. The protocol runs in two phases: a loading phase (2-2.5 mg twice weekly for 4-6 weeks), then maintenance (2 mg once weekly). The dose sizes are 10x larger than BPC-157 for the same tissue-repair category, which means the reco math, syringe choice, and vial counts all shift. This page covers both phases with a free calculator pre-filled for a typical maintenance dose.
Key Takeaways
- —TB-500 (TB4 fragment) ships in 5 mg or 10 mg lyo vials. 2 mL BAC water is the standard reco volume.
- —Standard reco: 5 mg vial + 2 mL BAC = 2.5 mg/mL. On a slin pin, 0.4 mL = 40 units = 1 mg dose.
- —Loading: 2-2.5 mg 2x/week for 4-6 weeks. Maintenance: 2 mg 1x/week. The Wolverine stack pairs this with BPC-157.
- —A 5 mg vial yields 2 maintenance doses (2 mg each); a 10 mg vial yields 5. Plan vial counts before you order.
- —Compared to BPC-157, TB-500 dosing is way less frequent (weekly vs daily) but each pin is bigger. See BPC-157 vs TB-500 for the mechanism split.
| Vial Size | BAC Water | Concentration | Notes |
|---|---|---|---|
| 2 mg | 1 mL | 2 mg/mL (2,000 mcg/mL) | Small vials, rare |
| 5 mg | 2 mL | 2.5 mg/mL (2,500 mcg/mL) | Most common; 1 mg = 40 units |
| 5 mg | 1 mL | 5 mg/mL (5,000 mcg/mL) | Smaller draw volume |
| 10 mg | 2 mL | 5 mg/mL (5,000 mcg/mL) | Most common 10 mg reco; 2 mg = 40 units |
| 10 mg | 5 mL | 2 mg/mL (2,000 mcg/mL) | Dilute; 2 mg = full 1 mL syringe |
The 5 mg + 2 mL reco is the practical default for the Khavinson-style 1 mg fractional doses, and 10 mg + 2 mL is the default for full 2-5 mg loading and maintenance work. Both keep most pins inside a single slin pin envelope. Swirl don't shake when the BAC water hits the lyo, TB-500 doesn't love mechanical shear.
Using the 5 mg vial + 2 mL BAC water = 2,500 mcg/mL standard reco math:
| Phase | Dose | Draw Volume | Slin Pin Units |
|---|---|---|---|
| Fractional | 1 mg | 0.4 mL | 40 units |
| Maintenance | 2 mg | 0.8 mL | 80 units |
| Loading (light) | 2.5 mg | 1.0 mL | 100 units (full slin pin) |
For the bigger doses people run on the 10 mg + 2 mL (5,000 mcg/mL) concentration:
| Phase | Dose | Draw Volume | Slin Pin Units |
|---|---|---|---|
| Maintenance | 2 mg | 0.4 mL | 40 units |
| Maintenance (high) | 2.5 mg | 0.5 mL | 50 units |
| Loading (light) | 4 mg | 0.8 mL | 80 units |
| Loading (standard) | 5 mg | 1.0 mL | 100 units (full slin pin) |
| Loading (heavy) | 6 mg | 1.2 mL | >100 units, switch to 3 mL syringe |
| Loading (max) | 10 mg | 2.0 mL | needs 3 mL syringe |
Loading doses above 5 mg blow past the capacity of a slin pin. Standard play: switch to a 1 mL tuberculin syringe for 4-6 mg pins, or split 8-10 mg into two sites. Rotate sites either way.
A TB-500 cycle runs in two phases:
Phase 1, Loading (4-6 weeks):
Phase 2, Maintenance (4-8 weeks):
Combined full cycle at the standard 2-2.5 mg loading + 2 mg maintenance protocols: ~25-40 mg of TB-500, or 3-4 x 10 mg vials over 8-14 weeks. Round up. Lyo holds at -20°C for years, extra vials aren't wasted, and you don't want to run short mid-cycle and reset the 28-day rule on a fresh reco.
Open the calculator pre-filled for the 2 mg maintenance dose on a 10 mg vial + 2 mL reco.
Other common deep-links:
No signup. Browser-based. Works on mobile. The calc shows draw volume in mL and slin pin units in real time as you adjust any input.
Both peptides sit in the tissue-repair category and most people run them together as the Wolverine stack. The dosing profiles are opposites:
| Dimension | BPC-157 | TB-500 |
|---|---|---|
| Typical dose | 250-500 mcg | 2-5 mg |
| Dose scale | 10-20x smaller | 10-20x larger |
| Frequency | Daily or 2x/day | Weekly (after loading) |
| Half-life basis | Short plasma, long PD | Long tissue residence |
| Standard vial | 5 mg | 10 mg |
| Slin pin units for typical dose | 10 | 40 |
BPC-157 is your localized repair signal, you pin it near the site (not in the joint) on a daily cadence. TB-500 is the systemic facilitator, weekly pins in the belly are fine because cell migration finds the injury wherever it is. Full mechanism comparison in BPC-157 vs TB-500.
Reco'd TB-500 holds 28 days at 2-8°C (regular fridge temp). Two rules:
For a 10 mg vial reco'd with 2 mL BAC water (5,000 mcg/mL): the 2 mg maintenance dose is 0.4 mL = 40 units on a slin pin. The 5 mg loading dose is 1.0 mL = a full slin pin. Switch to a 1 mL tuberculin syringe for cleaner measurement at the bigger loading doses.
Typical protocols run 8-14 weeks total: 4-6 weeks loading (twice-weekly pins of 2-2.5 mg, or 4-10 mg for heavier work) followed by 4-8 weeks maintenance (once-weekly 2 mg). Cycles past 16 weeks aren't common in practitioner protocols. See the TB-500 research guide for evidence context.
TB4 binds G-actin stoichiometrically, each molecule sequesters one actin monomer. The loading phase saturates available binding sites in tissue; maintenance dosing keeps the pool topped up. That's mechanistically different from BPC-157's pulsatile receptor activation, which is why BPC-157 doesn't need a loading phase.
Yep. A 10 mg vial reco'd with 2 mL BAC water gives you 5 maintenance doses (2 mg each) over ~5 weeks. Store at 2-8°C and use within 28 days of reco. Never freeze. Rotate sites with each pin.
Slin pins max out at 1 mL / 100 units. At the standard 5,000 mcg/mL concentration that's 5 mg. Pins above 5 mg need a 1 mL tuberculin syringe (for 6 mg) or 3 mL syringe (for 10 mg). The calculator flags when the calculated draw blows past 100 units.
This article is for research and informational purposes only. TB-500 is not FDA-approved for human clinical use. Consult a licensed healthcare professional before considering any peptide protocol.
Research Disclaimer. All content on Next Pep is for informational and educational purposes only. It does not constitute medical advice, diagnosis, or treatment. Consult a licensed healthcare professional before considering any peptide protocol.
CJC-1295 with DAC has a 6-8 DAY half-life; Modified GRF (1-29) clears in ~30 minutes. Same modified GHRH(1-29) backbone, one bolt-on linker, ~1,000x PK difference.
TB-500 is a 7-aa fragment of thymosin beta-4 (43 aa, ~4,963 Da), not the full protein. Cross-COA review: ~67% of "TB-500" vials are actually full Tβ4.